This ELISA (Enzyme-Linked Immunosorbent Assay) test is a qualitative serological method used to detect the presence of Alfalfa mosaic virus (AMV) in various ornamental and vegetable crops, providing a reliable tool for early diagnosis and disease management. AMV is a member of the Alfamovirus genus within the Bromoviridae family, and it is characterized by its bacilliform (rod-shaped) viral particles, which have a consistent diameter of approximately 18 nanometers. These viral particles contain a tripartite RNA genome and are transmitted primarily through aphid vectors in a non-persistent manner, making early detection crucial to prevent widespread outbreaks. The ELISA test works by using specific antibodies that bind to AMV antigens, allowing for the identification of infected plants even at low virus concentrations, thus aiding in effective monitoring and control strategies in agricultural practices.