This ELISA (Enzyme-Linked Immunosorbent Assay) test is a qualitative serological method designed to detect the presence of Cucumber mosaic virus (CMV) in plant tissues such as ornamental and vegetable leaves. The procedure involves using specific antibodies that bind to CMV antigens if present, allowing for the identification of infected samples. CMV, a member of the Cucumovirus genus, is characterized by its non-enveloped, spherical morphology, typically measuring around 28-30 nanometers in diameter. As a plant pathogen, CMV can cause a range of symptoms including mottling, distortion, and stunted growth, and it is known for its wide host range and ease of transmission through aphids and mechanical means. The ELISA test provides a reliable and efficient means of diagnosing CMV infections in plant material, facilitating early detection and management to prevent widespread crop damage.