The Fusarium Deoxynivalenol antigen test is a specialized competitive ELISA designed for semi-quantitative detection of Deoxynivalenol (DON), a mycotoxin produced by Fusarium species, in root sample material. This test works by utilizing antibodies that specifically bind to DON molecules, competing with the sample's toxin for binding sites on the antibody. When a root sample is applied, if DON is present, it will compete with a labeled antigen during the reaction, reducing the signal proportionally to the toxin concentration. The assay provides an estimate of DON levels in the sample, aiding in the assessment of fungal contamination and potential risks associated with mycotoxin presence. Its semi-quantitative nature offers insights into toxin levels, facilitating decisions related to crop safety and management practices.