This ELISA test is a qualitative serological assay specifically developed to detect Tobacco mosaic virus (TMV), a highly infectious pathogen causing Tobacco mosaic disease, which affects a wide range of crops including vegetables, fruits, and ornamental plants. TMV is a member of the Tobamovirus genus, known for its rigid, rod-shaped virions that are highly stable in the environment, facilitating its widespread transmission. The assay works by identifying the presence of TMV antigens in plant tissue samples through antibody-antigen interactions, providing a rapid and reliable method for disease diagnosis.