This ELISA (Enzyme-Linked Immunosorbent Assay) test is a qualitative serological technique used to detect the presence of Tobacco streak virus (TSV) in both ornamental and vegetable plant leaves. TSV is an Ilarvirus, a genus known for viruses with non-enveloped, quasi-spherical particles that are approximately 28-30 nanometers in diameter, and it primarily affects tobacco, ornamental plants, and certain vegetables. The test utilizes specific antibodies to identify viral antigens, providing a yes/no result indicating the presence or absence of TSV. Due to the structural similarities between TSV and Strawberry necrotic shock virus (SNSV), the test also shows cross-reactivity with SNSV, allowing it to serve as a diagnostic tool for detecting SNSV infections in plant tissues as well. This dual detection capability is particularly useful for plant disease management and epidemiological studies.